Difference between revisions of "Part:BBa K1933001"
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INPNC codes N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6×His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passengers. This domain do not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retains enzyme activity even when it is linked to INPNC with 6× His tag. | INPNC codes N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6×His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passengers. This domain do not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retains enzyme activity even when it is linked to INPNC with 6× His tag. | ||
For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>. | For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>. | ||
Revision as of 13:16, 10 October 2016
INPNC fused to 6xHis tag
INPNC codes N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6×His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passengers. This domain do not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retains enzyme activity even when it is linked to INPNC with 6× His tag. For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>.