Difference between revisions of "Part:BBa K1926003"
(No difference)
|
Revision as of 04:43, 10 October 2016
A cyclic promoter of CDK4 from human genome
This part is a cyclic promoter named CDK4 from human genome. It can lead to transcription of the downstream DNA sequence in every G1 phase[4].
4. Guo, Z.Y., et al., The elements of human cyclin D1 promoter and regulation involved. Clin Epigenetics, 2011. 2(2): p. 63-76.
You may use this part to:
1) Express something in mammal cell lines particularly in G1 phases or once in every cell cycle by stable transfecting it into cell line;
2) Use it as a human promoter by transient transfecting it into cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 522
Illegal NheI site found at 526 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 531
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 142
Illegal AgeI site found at 173 - 1000COMPATIBLE WITH RFC[1000]