Difference between revisions of "Part:BBa K2148007"

 
 
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The coding DNA sequence for Verde fluorescent protein with a HA Tag. This level-0 Phytobrick is coded as a CTAG Phytobrick, which allows it to be co-expressed with another CDS.
 
The coding DNA sequence for Verde fluorescent protein with a HA Tag. This level-0 Phytobrick is coded as a CTAG Phytobrick, which allows it to be co-expressed with another CDS.
  
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===Usage and Biology===
 
===Usage and Biology===
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This is a level-0 Phytobrick CTAG fluorescent protein. It can be used along with other level-0 Phytobrick promoters, CDS and 3UTR to create a transcriptional unit with a coexpressed vivid Verde fluorescent protein and a HA Tag.
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<partinfo>BBa_K2148007 parameters</partinfo>
 
<partinfo>BBa_K2148007 parameters</partinfo>
 
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===Characterisation===
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===References===
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Braun‐Galleani S, Baganz F, Purton S. Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid Verde Fluorescent Protein as a reporter. Biotechnology Journal. 2015;10(8):1289-1297. doi:10.1002/biot.201400566.
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Ilagan RP, Rhoades E, Gruber DF, Kao H-T, Pieribone VA, Regan L. A new bright green-emitting fluorescent protein: Engineered monomeric and dimeric forms. The FEBS journal. 2010;277(8):1967-1978. doi:10.1111/j.1742-4658.2010.07618.x.

Latest revision as of 23:59, 9 October 2016


Verde FP with HA Tag (CTAG Phytobrick)

The coding DNA sequence for Verde fluorescent protein with a HA Tag. This level-0 Phytobrick is coded as a CTAG Phytobrick, which allows it to be co-expressed with another CDS.


Usage and Biology

This is a level-0 Phytobrick CTAG fluorescent protein. It can be used along with other level-0 Phytobrick promoters, CDS and 3UTR to create a transcriptional unit with a coexpressed vivid Verde fluorescent protein and a HA Tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

References

Braun‐Galleani S, Baganz F, Purton S. Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid Verde Fluorescent Protein as a reporter. Biotechnology Journal. 2015;10(8):1289-1297. doi:10.1002/biot.201400566.

Ilagan RP, Rhoades E, Gruber DF, Kao H-T, Pieribone VA, Regan L. A new bright green-emitting fluorescent protein: Engineered monomeric and dimeric forms. The FEBS journal. 2010;277(8):1967-1978. doi:10.1111/j.1742-4658.2010.07618.x.