Difference between revisions of "Part:BBa K2148004"

 
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A level-0 Phytobrick coded as a promoter/5UTR with a Chlamydomonas reinhardtii chloroplast homology region upstream of the promoter sequence to allow for homologous recombination. The homology region is left from the trnE2-psbH integenic region in the Chlamydomonas reinhardtii chloroplast.
 
A level-0 Phytobrick coded as a promoter/5UTR with a Chlamydomonas reinhardtii chloroplast homology region upstream of the promoter sequence to allow for homologous recombination. The homology region is left from the trnE2-psbH integenic region in the Chlamydomonas reinhardtii chloroplast.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part can be used with another level-0 Phytobrick to create a transcriptional unit.
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<partinfo>BBa_K2148004 parameters</partinfo>
 
<partinfo>BBa_K2148004 parameters</partinfo>
 
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===Characterisation===
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===References===
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Wannathong T, Waterhouse JC, Young REB, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2016;100:5467-5477. doi:10.1007/s00253-016-7354-6.

Revision as of 23:37, 9 October 2016


Homology-psaA promoter/5UTR/NTAG

A level-0 Phytobrick coded as a promoter/5UTR with a Chlamydomonas reinhardtii chloroplast homology region upstream of the promoter sequence to allow for homologous recombination. The homology region is left from the trnE2-psbH integenic region in the Chlamydomonas reinhardtii chloroplast.


Usage and Biology

This part can be used with another level-0 Phytobrick to create a transcriptional unit.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

References

Wannathong T, Waterhouse JC, Young REB, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2016;100:5467-5477. doi:10.1007/s00253-016-7354-6.