Difference between revisions of "Part:BBa K2148002"

 
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This is the coding DNA sequence that confers antibiotic resistance to amikacin, neomycin, and kanamycin. The CDS is flanked by Phytobrick fusion sites which corresponds to a CDS/CTAG Phytobrick.
 
This is the coding DNA sequence that confers antibiotic resistance to amikacin, neomycin, and kanamycin. The CDS is flanked by Phytobrick fusion sites which corresponds to a CDS/CTAG Phytobrick.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part can be used with other level 0 Phytobrick to create a transcriptional unit to provide a selection pressure on transformations.
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<partinfo>BBa_K2148002 parameters</partinfo>
 
<partinfo>BBa_K2148002 parameters</partinfo>
 
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===Characterization===
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===References===
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Bateman J. M., Purton S. (2000). Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker. Mol. Gen. Genet. 263, 404–410. 10.1007/s004380051184

Revision as of 23:17, 9 October 2016


aphA6

This is the coding DNA sequence that confers antibiotic resistance to amikacin, neomycin, and kanamycin. The CDS is flanked by Phytobrick fusion sites which corresponds to a CDS/CTAG Phytobrick.


Usage and Biology

This part can be used with other level 0 Phytobrick to create a transcriptional unit to provide a selection pressure on transformations.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 647
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 647
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 647
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 647
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

References

Bateman J. M., Purton S. (2000). Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker. Mol. Gen. Genet. 263, 404–410. 10.1007/s004380051184