Difference between revisions of "Part:BBa K2092004:Design"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2092005 short</partinfo>
+
<partinfo>BBa_K2092004 short</partinfo>
  
−
<partinfo>BBa_K2092005 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2092004 SequenceAndFeatures</partinfo>
−
 
+
−
 
+
−
===Design Notes===
+
−
- Codon optimized the sequence retrieved from the NCBI database (original sequence is of eukaryotic origin).
+
−
- Calculated the RBS strength using the SalisLab RBS Calculator.
+
  
  
 +
Calculated the RBS strength by using the online tool SalisLab RBS Calculator.
  
 
===Source===
 
===Source===
  
−
Aspergillus nidulans, sequence found through <a href="https://www.ncbi.nlm.nih.gov/protein/40745154"> NCBI Database </a>
+
<i>Aspergillus nidulans.</i> The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a> [1]. This version is codon optimized for E coli by Thermo Fisher Scientific and synthesized by IDT.
  
 
===References===
 
===References===
 +
 +
[1] Felenbok,B., Sequeval,D., Mathieu,M., Sibley,S., Gwynne,D.I. & Davies,R.W. (1988). The ethanol regulon in Aspergillus nidulans: characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385-396.

Latest revision as of 22:32, 9 October 2016


alcR (incl RBS), ethanol-activated transcription factor from A. nidulans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2568
    Illegal BamHI site found at 1572
    Illegal BamHI site found at 2310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1276
    Illegal AgeI site found at 1404
    Illegal AgeI site found at 1935
  • 1000
    COMPATIBLE WITH RFC[1000]


Calculated the RBS strength by using the online tool SalisLab RBS Calculator.

Source

Aspergillus nidulans. The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a> [1]. This version is codon optimized for E coli by Thermo Fisher Scientific and synthesized by IDT.

References

[1] Felenbok,B., Sequeval,D., Mathieu,M., Sibley,S., Gwynne,D.I. & Davies,R.W. (1988). The ethanol regulon in Aspergillus nidulans: characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385-396.