Difference between revisions of "Part:BBa K2132008:Design"

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===Design Notes===
 
===Design Notes===
 
We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.
 
We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.
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===Source===
 
===Source===
  
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The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.
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The sequence of '' hydG '' hydrogenase gene is from the chromosome of '' Clostridium acetobutylicum '' ATCC 824.
  
 
===References===
 
===References===
  
 
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.
 
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.

Latest revision as of 13:14, 9 October 2016


HydG with Histag and TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 685
    Illegal AgeI site found at 982
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 418
    Illegal BsaI.rc site found at 646
    Illegal SapI.rc site found at 57


Design Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

Source

The sequence of hydG hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

References

Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.