Difference between revisions of "Part:BBa K2132006:Design"
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===Source=== | ===Source=== | ||
| − | The sequence of hydE hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. | + | The sequence of '' hydE '' hydrogenase gene is from the '' chromosome '' of Clostridium acetobutylicum ATCC 824. |
===References=== | ===References=== | ||
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72. | Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72. | ||
Revision as of 13:02, 9 October 2016
HydE with Histag and TEV site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 558
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 526
Illegal SapI.rc site found at 324
Design Notes
We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.
Source
The sequence of hydE hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.
References
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.