Difference between revisions of "Part:BBa K2132008:Design"

Revision as of 12:42, 9 October 2016

HydG with Histag and TEV site

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 685
Illegal AgeI site found at 982
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 418
Illegal BsaI.rc site found at 646
Illegal SapI.rc site found at 57

Design Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

Source

The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

References

Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.

Revision as of 12:03, 9 October 2016 (view source) Registry (Talk \| contribs)		Revision as of 12:42, 9 October 2016 (view source) Chenyf (Talk \| contribs) (→‎References) Newer edit →
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	===References===		===References===
		+
		+	Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.