Difference between revisions of "Part:BBa K2132006:Design"

(→‎References)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.
 
We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.
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===Source===
 
===Source===

Revision as of 12:40, 9 October 2016


HydE with Histag and TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 526
    Illegal SapI.rc site found at 324


Design Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

Source

The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

References

Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.