Difference between revisions of "Part:BBa K2132006:Design"

Revision as of 12:13, 9 October 2016

HydE with Histag and TEV site

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 558
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 526
Illegal SapI.rc site found at 324

Design Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

Source

The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

References

Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.

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	===References===		===References===
		+	Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.