Difference between revisions of "Part:BBa K2066030"

Line 1: Line 1:
 +
pTAC Ribozyme Characterization Part – GFP only; no RiboJ
 +
 +
 
This part was designed to be assembled via Gibson Assembly from WM16_014 (using primers WM16_P027 and WM16_P009) and the same WM16_014 again (using primers WM16_P019 and WM16_P025).
 
This part was designed to be assembled via Gibson Assembly from WM16_014 (using primers WM16_P027 and WM16_P009) and the same WM16_014 again (using primers WM16_P019 and WM16_P025).
  
 
This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
 
This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.

Revision as of 03:50, 9 October 2016

pTAC Ribozyme Characterization Part – GFP only; no RiboJ


This part was designed to be assembled via Gibson Assembly from WM16_014 (using primers WM16_P027 and WM16_P009) and the same WM16_014 again (using primers WM16_P019 and WM16_P025).

This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.