Difference between revisions of "Part:BBa K2151007"

Revision as of 21:40, 8 October 2016

p25-I13500: GFP under control of medium constitutive promoter and strong ribosome binding site

This part consists of I13500 (GFP with a strong ribosome binding site) under the control of p25, a medium constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p25 was initially identified in a promoter probe survey of the Streptococcus thermophilus genome, alongside weaker promoters p8 and p20.

We used p25 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p25 using GFP in E. coli revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 707

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 <partinfo>BBa_K2151007 short</partinfo>
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+<p>This part consists of I13500 (GFP with a strong ribosome binding site) under the control of p25, a medium constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p25 was initially identified in a promoter probe survey of the <i>Streptococcus thermophilus</i> genome, alongside weaker promoters p8 and p20.</p>
+ <p>We used p25 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p25 using GFP in <i>E. coli</i> revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).</p>
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