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Latest revision as of 20:43, 11 June 2007


feruloyl-CoA hydratase for vanillin biosynthesis (ech)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The TGA stop codon was mutated to TAATAA to conform with BioBrick protein coding region standards.

There are two BioBrick restriction sites in this coding region that we need to mutate.

Source

The natural source for this genetic material is Pseudomonas fluorescens; see Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, Barghini et al. 2007

Genbank accession number AJ536325 (GI 40788116)


References