Difference between revisions of "Part:BBa K2020000:Design"
 
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===Design Notes===
 
===Design Notes===
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The sequence from ''B. subtilis'' was codon optimized for ''E. coli'' with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.
 
  
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The sequence from ''B. subtilis'' was codon optimized for ''E. coli'' with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.
  
  
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The sequence was originally obtained from a wildtype ''Bacillus subtilis'' and was ordered from IDT.
 
The sequence was originally obtained from a wildtype ''Bacillus subtilis'' and was ordered from IDT.
  
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===References===
 
===References===
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Latest revision as of 20:08, 8 October 2016


subtilisin E gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence from B. subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.


Source

The sequence was originally obtained from a wildtype Bacillus subtilis and was ordered from IDT.