Difference between revisions of "Part:BBa K2020001"
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<partinfo>BBa_K2020001 short</partinfo>
 
<partinfo>BBa_K2020001 short</partinfo>
  
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This composite part consists of the leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) and the subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]). It can be used to express subtilisin E in ''Escherichia coli'' and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
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This DNA sequence can be used to express subtilisin E in ''Escherichia coli'' and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
  
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2020001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2020001 SequenceAndFeatures</partinfo>

Revision as of 20:05, 8 October 2016


subtilisin E gene with leader sequence pelB

This DNA sequence can be used to express subtilisin E in Escherichia coli and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]