Difference between revisions of "Part:BBa K2042008"

 
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<partinfo>BBa_K2042008 short</partinfo>
 
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This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor
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This is the coding sequence of D-lactate deshydrogenase from Lactobacillus delbrueckii 11842. The sequence was condon optimized for Pseudomonas putida and point mutations on three amino acids (D176S, I177R, F178T) were found to efficiently use both NADH and NADPH as cofactors. This enzyme would be useful, as the activity of such an enzyme will not be limited by the availability of NADH or NADPH.
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<partinfo>BBa_K2042008 parameters</partinfo>
 
<partinfo>BBa_K2042008 parameters</partinfo>
 
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== Predicted structure==
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[[File:BBa_K2042008_model.gif]]
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== References: ==
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Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.<br>
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Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2.<br>
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https://www.ncbi.nlm.nih.gov/pubmed/27109778

Latest revision as of 19:18, 8 October 2016


Engineered D-Lactate dehydrogenase (D-LDH*)

This is the coding sequence of D-lactate deshydrogenase from Lactobacillus delbrueckii 11842. The sequence was condon optimized for Pseudomonas putida and point mutations on three amino acids (D176S, I177R, F178T) were found to efficiently use both NADH and NADPH as cofactors. This enzyme would be useful, as the activity of such an enzyme will not be limited by the availability of NADH or NADPH.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]


Predicted structure

BBa K2042008 model.gif

References:

Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.
Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2.
https://www.ncbi.nlm.nih.gov/pubmed/27109778