Difference between revisions of "Part:BBa J100205:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pSB1A8 with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix.
+
The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pUC-IDT-Amp (BsaI site removed) with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix.
  
  

Latest revision as of 16:10, 8 October 2016


repClone Red


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2288
    Illegal AgeI site found at 2400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638
    Illegal BsaI.rc site found at 1517


Design Notes

The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pUC-IDT-Amp (BsaI site removed) with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix.



Source

The two main indicator proteins are GFP (BBa_I746916) and RFP (BBa_E1010).


References