Difference between revisions of "Part:BBa K1957001"

Revision as of 14:59, 8 October 2016

HydC subunit of FeFe Hydrogenase

One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydC subunit, which is the outer periplasmic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.

Figure 1: PCR Colony of FeFe Hydrogenase subunits. Lanes 8 and 9 have HydC/Fdh (606) gene insert, which is 764bp.

DNA of the colony on lane 8 was sent off for sequencing. After sequencing confirmation it was used for the Golden Gate cloning and submitted into the iGEM registry.

Restriction site incompatibility, [1000], is with regards to MoClo i.e. Golden Gate cloning. As we used this in downstream cloning experiments the restriction site could not be avoided.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 700

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 One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydC subunit, which is the outer periplasmic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.
-[[Image:T--NRP-UEA-Norwich--Gel_PCR_Colony_FeFe_.jpg|thumb|Figure 1: PCR Colony of FeFe Hydrogenase subunits. Lanes 8 and 9 have HydC/Fdh (606) gene insert, which is 764bp.]] DNA of the colony on lane 8 was sent off for sequencing. After sequencing confirmation it was used for the Golden Gate cloning and submitted into the iGEM registry.
+[[File:T--NRP-UEA-Norwich--Gel_PCR_Colony_FeFe_.jpg|thumb|Figure 1: PCR Colony of FeFe Hydrogenase subunits. Lanes 8 and 9 have HydC/Fdh (606) gene insert, which is 764bp.]] DNA of the colony on lane 8 was sent off for sequencing. After sequencing confirmation it was used for the Golden Gate cloning and submitted into the iGEM registry.
 Restriction site incompatibility, [1000], is with regards to MoClo i.e. Golden Gate cloning. As we used this in downstream cloning experiments the restriction site could not be avoided.