Difference between revisions of "Part:BBa K2010002"

 
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<partinfo>BBa_K2010002 short</partinfo>
 
<partinfo>BBa_K2010002 short</partinfo>
  
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This GGS linker is a linker sequence that neutrally connects two protein domains together.
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PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
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The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein.
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This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification. In addition, this part includes pelB, an excretion tag that transports proteins to the periplasm.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 06:51, 8 October 2016


pelB + PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification. In addition, this part includes pelB, an excretion tag that transports proteins to the periplasm. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 112
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 528