Difference between revisions of "Part:BBa K2114998:Design"

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===References===
 
===References===
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Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401

Revision as of 00:33, 7 October 2016


pelB_aGFPnano


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure the formation of the characteristic disulfide bond of the nanobody the pelB leader sequence was included in the N-terminus of the nanobody by primer extensions and Gibson assembly.


Source

The anti-GFP nanobody is derived camelid heavy-chain antibodies and was provided by Dr. Maximilian Ulbrich (BIOSS).

References

Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401