Difference between revisions of "Part:BBa K2052014"

Revision as of 16:26, 6 October 2016

FimH site directed mutated with RPMrel

Usage and Biology: Some substrains of E.Coli have a structure called Type 1 pili which is expressed from the Fim gene system. At the end of the pili structure there is a protein called “FimH” which is the structure that allows them to bind to the mannose sugar that is found on the surfaces of eukaryotic cells. (Sauer et al., 2016).However, the substrain that was used in this project was BL21, a non-pathogenic laboratory strain. Deleted mannose binding and replacing it with RPMrel would provide tumor specific binding (Kelly et al., 2003).The CPIEDRPMC (RPMrel) peptide can bind to five colon cancer cell lines: HT29, CaCo-2, RKO, SW480, and DLD-1. Here on we have choosen CaCo-2 that is studied commonly in METU as our candidate to show targeted thearpy.

3D Structure of FimH together with RPMrel can be seen below as Harvard BioDesign 2015 submitted in part registry

....Enter a long description of the part so that users of your part know what it is, what it does, and how to use it in their projects.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1173
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1113
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
-Function:
+Usage and Biology:
-Some substrains of E.Coli have structure called Type 1 pili which is expressed from the Fim gene system. At the end of the pili structure there is a protein  called “FimH” which is the structe that allows them to bind to the mannose sugar that is found on the surfaces of eukaryotic cells. (Sauer et al., 2016).However, the substrain that was used in this project was BL21, a non-pathogenic substrain. So, it does not bind to eukaryotic cells.
+Some substrains of E.Coli have a structure called Type 1 pili which is expressed from the Fim gene system. At the end of the pili structure there is a protein called “FimH” which is the structure that allows them to bind to the mannose sugar that is found on the surfaces of eukaryotic cells. (Sauer et al., 2016).However, the substrain that was used in this project was BL21, a non-pathogenic laboratory strain. Deleted mannose binding and replacing it with RPMrel would provide tumor specific binding (Kelly et al., 2003).The CPIEDRPMC (RPMrel) peptide can bind to five colon cancer cell lines: HT29, CaCo-2, RKO, SW480, and DLD-1. Here on we have choosen CaCo-2 that is studied commonly in METU as our candidate to show targeted thearpy.
-Rpmrel, is a tumor specific binding peptide (Kelly et al., 2003). When combined with FimH it can bind to the tumor cells in the colon flora.
+D Structure of FimH together with RPMrel can be seen below as Harvard BioDesign 2015 submitted  in part registry
+[[File:METU_HS_2014partinagif.gif]]
 ....Enter a long description of the part so that users of your part know what it is, what it does, and how to use it in their projects.