Difference between revisions of "Part:BBa K1959000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Sequence has been optimized for expression in Orazy sativa.
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Sequence has been optimized for expression in Orazy sativa. Nucleotide substitution: g117c, gc195cg, g372a, c529a, t753a, a769t, a798g, g819a, t927c, a1031c. The numbers stand for the location where nucleotide subititution carried out. Nucleotide fellow the numbers is the mutant result.
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Latest revision as of 03:57, 6 October 2016


Modification of Phytoene synthase (PSY)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1045
    Illegal NheI site found at 1164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 105
    Illegal AgeI site found at 956
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence has been optimized for expression in Orazy sativa. Nucleotide substitution: g117c, gc195cg, g372a, c529a, t753a, a769t, a798g, g819a, t927c, a1031c. The numbers stand for the location where nucleotide subititution carried out. Nucleotide fellow the numbers is the mutant result.


Source

The complete CDS sequence can be obtained from Genebank. Genebank accession:U32636

References