Difference between revisions of "Part:BBa K1959001"
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<partinfo>BBa_K1959001 short</partinfo> | <partinfo>BBa_K1959001 short</partinfo> | ||
| − | This part is the coding sequence of phytoene desaturase from Erwinia uredovora. Phytoene desaturase is part of carotenoid biosynthesis pathway and it takes responsible for convertion of phytoene to lycopene. Codon optimized has been made for the expression in | + | This part is the coding sequence of phytoene desaturase from Erwinia uredovora. Phytoene desaturase is part of carotenoid biosynthesis pathway and it takes responsible for convertion of phytoene to lycopene. A Pea transit peptide is fused with cds of phytoene desaturase in order to stablize and help phytoene desaturase folding in a correct formation in Orazy sativa cell. Codon optimized has been made for the expression in Orazy sativa. |
| − | Orazy sativa. | + | |
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Revision as of 03:41, 6 October 2016
Modification of phytoene desaturase (PDS/CrtI)
This part is the coding sequence of phytoene desaturase from Erwinia uredovora. Phytoene desaturase is part of carotenoid biosynthesis pathway and it takes responsible for convertion of phytoene to lycopene. A Pea transit peptide is fused with cds of phytoene desaturase in order to stablize and help phytoene desaturase folding in a correct formation in Orazy sativa cell. Codon optimized has been made for the expression in Orazy sativa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 242
Illegal XhoI site found at 1372 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1582
- 1000COMPATIBLE WITH RFC[1000]