Difference between revisions of "Part:BBa K2020002"

Revision as of 17:23, 5 October 2016

expression system for subtilisin E in E. coli

This composite part consists of the promoter BBa_R0010, the ribosome binding site BBa_B0034, the newly created BioBrick part BBa_K2020001 and the terminator BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (BBa_J32015) and a subtilisin E gene optimized for Escherichia coli codon usage (BBa_K2020000). Once introduced into E. coli, this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 280
1000
COMPATIBLE WITH RFC[1000]

Revision as of 17:03, 5 October 2016 (view source) NicolaFreyer (Talk \| contribs) ← Older edit		Revision as of 17:23, 5 October 2016 (view source) NicolaFreyer (Talk \| contribs) Newer edit →
Line 3:		Line 3:
	<partinfo>BBa_K2020002 short</partinfo>		<partinfo>BBa_K2020002 short</partinfo>

−	This composite part consists of the promoter [[Part:BBa_R0010\|BBa_R0010]], the ~~RBS~~ [[Part:BBa_B0034\|BBa_B0034]], the newly created BioBrick part [[Part:BBa_K2020001\|BBa_K2020001]] and the terminator [[Part:BBa_B0010\|BBa_B0010]]. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB ([[Part:BBa_J32015\|BBa_J32015]]) and a subtilisin E gene optimized for ''Escherichia coli'' codon usage ([[Part:BBa_K2020000\|BBa_K2020000]]). Once introduced into ''E. coli'', this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.	+	This composite part consists of the promoter [[Part:BBa_R0010\|BBa_R0010]], the ribosome binding site [[Part:BBa_B0034\|BBa_B0034]], the newly created BioBrick part [[Part:BBa_K2020001\|BBa_K2020001]] and the terminator [[Part:BBa_B0010\|BBa_B0010]]. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB ([[Part:BBa_J32015\|BBa_J32015]]) and a subtilisin E gene optimized for ''Escherichia coli'' codon usage ([[Part:BBa_K2020000\|BBa_K2020000]]). Once introduced into ''E. coli'', this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.