Difference between revisions of "Part:BBa K2020002"

 
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<partinfo>BBa_K2020002 short</partinfo>
 
<partinfo>BBa_K2020002 short</partinfo>
  
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This composite part consists of the promoter [https://parts.igem.org/Part:BBa_R0010 BBa_R0010], the RBS [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], the newly created BioBrick part [https://parts.igem.org/Part:BBa_K2020001 BBa_K2020001] and the terminator [https://parts.igem.org/Part:BBa_B0010 BBa_B0010]. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB ([https://parts.igem.org/Part:BBa_J32015 BBaJ32015]) and a subtilisin E gene optimized for E. coli codon usage ([https://parts.igem.org/Part:BBa_K2020000 BBa_K2020000]). Once introduced into Escherichia coli, this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
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This composite part consists of the promoter [https://parts.igem.org/Part:BBa_R0010 BBa_R0010], the RBS [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], the newly created BioBrick part [https://parts.igem.org/Part:BBa_K2020001 BBa_K2020001] and the terminator [https://parts.igem.org/Part:BBa_B0010 BBa_B0010]. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB ([https://parts.igem.org/Part:BBa_J32015 BBa_J32015]) and a subtilisin E gene optimized for ''Escherichia coli'' codon usage ([https://parts.igem.org/Part:BBa_K2020000 BBa_K2020000]). Once introduced into ''E. coli'', this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
  
  

Revision as of 16:42, 5 October 2016


expression system for subtilisin E in E. coli

This composite part consists of the promoter BBa_R0010, the RBS BBa_B0034, the newly created BioBrick part BBa_K2020001 and the terminator BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (BBa_J32015) and a subtilisin E gene optimized for Escherichia coli codon usage (BBa_K2020000). Once introduced into E. coli, this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    COMPATIBLE WITH RFC[1000]