Difference between revisions of "Part:BBa K1913007:Design"

 
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===Source===
 
===Source===
  
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This part was Gibson assembled from fragment originating from biobrick parts BBa_I0500, BBa_C0052, and BBa_K081007.
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This part was Gibson assembled from fragments originating from biobrick parts BBa_I0500, BBa_C0052, and BBa_K081007.
  
 
===References===
 
===References===

Revision as of 11:35, 3 October 2016


434- and lambda cI operon for tuning protein balance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1898
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The LVA tag encoded by the lambda cI gene was purposefully left out of this part to provide different turnover rates for both cI proteins. This allow regulating the balance between the proteins based on changes in promoter strength over time.


Source

This part was Gibson assembled from fragments originating from biobrick parts BBa_I0500, BBa_C0052, and BBa_K081007.

References