Difference between revisions of "Part:BBa K2036024:Design"

(→‎Design Notes)
(→‎Source)
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iLDH are from the Registry and beta-galactosidase coding gene are referred to pUC119 commerial vector and synthesised by IDT.
 
iLDH are from the Registry and beta-galactosidase coding gene are referred to pUC119 commerial vector and synthesised by IDT.
 +
<br>CI,CII,CIII,Cro,pRE,pR,pRM are from bacteriaphage lambda.
  
 
===References===
 
===References===

Revision as of 09:44, 3 October 2016


placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3212
    Illegal BamHI site found at 3251
    Illegal BamHI site found at 3943
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a lactic acid balance circuit in E.coli with lactose signal to initiate. And when the pH environment are within 7 to 9,the host strain will prepare beta-galactosidase for next lactose input.
beta-galactosidase coding gene are alpha domain of laz,it functions only in E.coli strain which contains lacZ operon.

Source

iLDH are from the Registry and beta-galactosidase coding gene are referred to pUC119 commerial vector and synthesised by IDT.
CI,CII,CIII,Cro,pRE,pR,pRM are from bacteriaphage lambda.

References