Difference between revisions of "Part:BBa K2036033:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | PP2CA can dephosphorylate SnRK2.2 to quikly shut down it activity. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | SnRK2.2 and PP2CA are both from Arabidopsis thaliana ABA response pathway. | |
===References=== | ===References=== | ||
| + | Tim Lynch • B. Joy Erickson • Ruth R. Finkelstein(2012) Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response. Plant Mol Biol. 2012 Dec;80(6):647-58. doi: 10.1007/s11103-012-9973-3. Epub 2012 Sep 25.<br> | ||
| + | Fen-Fen Soon, Ley-Moy Ng, X. Edward Zhou, Graham M. West, Amanda Kovach, M. H. Eileen Tan, Kelly M. Suino-Powell,Yuanzheng He, Yong Xu, Michael J. Chalmers, Joseph S. Brunzelle, Huiming Zhang（2012）Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases. Science. 2012 Jan 6;335(6064):85-8. doi: 10.1126/science.1215106. Epub 2011 Nov 24. | ||
Latest revision as of 08:47, 3 October 2016
PP2CA-TTADH1-pCyc-Kozak-SnRK2.2-TTADH1-pCyc
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 90
Illegal BamHI site found at 661
Illegal BamHI site found at 806
Illegal BamHI site found at 918
Illegal BamHI site found at 1816
Illegal BamHI site found at 2473
Illegal XhoI site found at 65
Illegal XhoI site found at 875 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 48
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PP2CA can dephosphorylate SnRK2.2 to quikly shut down it activity.
Source
SnRK2.2 and PP2CA are both from Arabidopsis thaliana ABA response pathway.
References
Tim Lynch • B. Joy Erickson • Ruth R. Finkelstein(2012) Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response. Plant Mol Biol. 2012 Dec;80(6):647-58. doi: 10.1007/s11103-012-9973-3. Epub 2012 Sep 25.
Fen-Fen Soon, Ley-Moy Ng, X. Edward Zhou, Graham M. West, Amanda Kovach, M. H. Eileen Tan, Kelly M. Suino-Powell,Yuanzheng He, Yong Xu, Michael J. Chalmers, Joseph S. Brunzelle, Huiming Zhang（2012）Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases. Science. 2012 Jan 6;335(6064):85-8. doi: 10.1126/science.1215106. Epub 2011 Nov 24.