Difference between revisions of "Part:BBa K2036023"
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<partinfo>BBa_K2036023 short</partinfo> | <partinfo>BBa_K2036023 short</partinfo> | ||
| − | This part is built by In-Fusion cloning method and together with [https://parts.igem.org/Part:BBa_K2036021 BBa_K2036021] and [https://parts.igem.org/Part:BBa_K2036023 BBa_K2036023] to further assemble [https://parts.igem.org/Part:BBa_K2036024 BBa_K2036024] | + | This part is built by In-Fusion cloning method and together with [https://parts.igem.org/Part:BBa_K2036021 BBa_K2036021] and [https://parts.igem.org/Part:BBa_K2036023 BBa_K2036023] to further assemble [https://parts.igem.org/Part:BBa_K2036024 BBa_K2036024]. pRM can be negatively regulated by Cro. ilDH and beta-glactosidase are important enzyme in celluler metobolism. |
[[File:T--HUST-China--Experiments-Fig15.png|thumb|350px|center|Fig1: Prokaryote application plasmid for lactose intolerance]] | [[File:T--HUST-China--Experiments-Fig15.png|thumb|350px|center|Fig1: Prokaryote application plasmid for lactose intolerance]] | ||
Revision as of 08:43, 3 October 2016
RBS-iLDH-TT-pRM-RBS-beta-galactosidase
This part is built by In-Fusion cloning method and together with BBa_K2036021 and BBa_K2036023 to further assemble BBa_K2036024. pRM can be negatively regulated by Cro. ilDH and beta-glactosidase are important enzyme in celluler metobolism.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 737
Illegal BamHI site found at 776
Illegal BamHI site found at 1468 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]