Difference between revisions of "Part:BBa K2092005:Design"

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===Design Notes===
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Calculated the RBS strength by using the online tool SalisLab RBS Calculator.
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- Codon optimized the sequence retrieved from the NCBI database.
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- Calculated the RBS strength by using the online tool SalisLab RBS Calculator.
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===Source===
 
===Source===
  
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<i>Aspergillus nidulans.</i> The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a>. This version is codon optimized for E coli by Thermo Fisher Scientific.
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<i>Aspergillus nidulans.</i> The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a> [1]. This version is codon optimized for E coli by Thermo Fisher Scientific and synthesized by IDT.
  
 
===References===
 
===References===
  
 
[1] Felenbok,B., Sequeval,D., Mathieu,M., Sibley,S., Gwynne,D.I. & Davies,R.W. (1988). The ethanol regulon in Aspergillus nidulans: characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385-396.
 
[1] Felenbok,B., Sequeval,D., Mathieu,M., Sibley,S., Gwynne,D.I. & Davies,R.W. (1988). The ethanol regulon in Aspergillus nidulans: characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385-396.

Latest revision as of 22:44, 2 October 2016


alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2568
    Illegal BamHI site found at 1572
    Illegal BamHI site found at 2310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1276
    Illegal AgeI site found at 1404
    Illegal AgeI site found at 1935
  • 1000
    COMPATIBLE WITH RFC[1000]


Calculated the RBS strength by using the online tool SalisLab RBS Calculator.

Source

Aspergillus nidulans. The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a> [1]. This version is codon optimized for E coli by Thermo Fisher Scientific and synthesized by IDT.

References

[1] Felenbok,B., Sequeval,D., Mathieu,M., Sibley,S., Gwynne,D.I. & Davies,R.W. (1988). The ethanol regulon in Aspergillus nidulans: characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385-396.