Difference between revisions of "Part:BBa K2092005:Design"
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===Design Notes=== | ===Design Notes=== | ||
- Codon optimized the sequence retrieved from the NCBI database. | - Codon optimized the sequence retrieved from the NCBI database. | ||
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- Calculated the RBS strength by using the online tool SalisLab RBS Calculator. | - Calculated the RBS strength by using the online tool SalisLab RBS Calculator. | ||
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===Source=== | ===Source=== | ||
Revision as of 21:26, 2 October 2016
alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2568
Illegal BamHI site found at 1572
Illegal BamHI site found at 2310 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1276
Illegal AgeI site found at 1404
Illegal AgeI site found at 1935 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Codon optimized the sequence retrieved from the NCBI database.
- Calculated the RBS strength by using the online tool SalisLab RBS Calculator.
Source
Aspergillus nidulans, sequence found through