Difference between revisions of "Part:BBa K2010001"

Revision as of 19:27, 2 October 2016

ompT + PETase (PET-degrading enzyme, origin I. sakaiensis) PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification. In addition, this part includes ompT, an excretion tag that transports proteins to the extracellular medium.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 522

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 __NOTOC__
 <partinfo>BBa_K2010001 short</partinfo>
+PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
-MHETase is the MHET(Mono(2-hydroxyethyl) terephthalate)-degrading enzyme first identified in Ideonella sakaiensis.
+This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification.
-This sequence is the E. coli K12 optimized DNA sequence for MHETase.
+In addition, this part includes ompT, an excretion tag that transports proteins to the extracellular medium.
 <!-- Add more about the biology of this part here