Difference between revisions of "Part:BBa K2042008:Design"
RATOVOMANANA (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T) | + | The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T) to efficiently use both NADH and NADPH as cofactors. Silent mutation were also introduced for removing restriction site. |
===Source=== | ===Source=== | ||
Latest revision as of 20:21, 1 October 2016
Engineered D-Lactate dehydrogenase (D-LDH*)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 630
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T) to efficiently use both NADH and NADPH as cofactors. Silent mutation were also introduced for removing restriction site.
Source
Synthetic synthesis