Difference between revisions of "Part:BBa K2042008:Design"

(Design Notes)
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
This enzyme is for increasing lactate
+
The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T)
  
 
===Source===
 
===Source===

Revision as of 18:20, 1 October 2016


Engineered D-Lactate dehydrogenase (D-LDH*)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T)

Source

Synthetic synthesis

References