Difference between revisions of "Part:BBa K2092002:Design"

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===Design Notes===
 
===Design Notes===
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To add the missing XbaI and SpeI restriction sites, the following two primers were designed:
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XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark.  To add the following sites, two primers were designed:
 
alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3'  
 
alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3'  
 
alcA reverse primer =  
 
alcA reverse primer =  
 
5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'
 
5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'
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===Source===
 
===Source===

Revision as of 16:02, 1 October 2016


PalcA, improved alcR inducible promoter from A. nidulans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark. To add the following sites, two primers were designed: alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3' alcA reverse primer = 5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'

Source

Aspergillus nidulans, sequence found through

References