Difference between revisions of "Part:BBa K2036010"
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It is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example,after constructing PETDurt-1-Cro-TT-pRM-RBS-GFP-LVAssrAtag,IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator. | It is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example,after constructing PETDurt-1-Cro-TT-pRM-RBS-GFP-LVAssrAtag,IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator. | ||
| − | HUST-Chian 2016 Build this circuit to test Cro and pRM interaction intensity with contol group:pRM-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036009 BBa_K2036009]) | + | <br>HUST-Chian 2016 Build this circuit to test Cro and pRM interaction intensity with contol group:pRM-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036009 BBa_K2036009]) |
[[File:T--HUST-China--Experiments-Fig12.png|thumb|400px|center|Fig1:Cro&pRM interaction characterization circuit]] | [[File:T--HUST-China--Experiments-Fig12.png|thumb|400px|center|Fig1:Cro&pRM interaction characterization circuit]] | ||
Revision as of 07:44, 28 September 2016
Cro-TT-pRM-RBS-GFP-LVAssrAtag
It is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example,after constructing PETDurt-1-Cro-TT-pRM-RBS-GFP-LVAssrAtag,IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
HUST-Chian 2016 Build this circuit to test Cro and pRM interaction intensity with contol group:pRM-GFP-LVAssrAtag (BBa_K2036009)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1036