Difference between revisions of "Part:BBa K2036009"
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<partinfo>BBa_K2036009 short</partinfo>
 
<partinfo>BBa_K2036009 short</partinfo>
  
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pRM-GFP-LVAtag, the circuit can function as control group of Cro and pRM interaction characterization comparing to Cro-TT-pRM-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K20360010 BBa_K20360010]).
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pRM-GFP-LVAtag, the circuit can function as control group of Cro and pRM interaction characterization with test group: Cro-TT-pRM-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K20360010 BBa_K20360010]).
 
<br>When inserted into expression plasmid and tramsformed into E.coli. Take PETDuet-1 as an example, IPTG can trigger T7 promoter in the plasmid, and  cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
 
<br>When inserted into expression plasmid and tramsformed into E.coli. Take PETDuet-1 as an example, IPTG can trigger T7 promoter in the plasmid, and  cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
  

Revision as of 07:32, 28 September 2016


pRM-GFP-LVAssrAtag

pRM-GFP-LVAtag, the circuit can function as control group of Cro and pRM interaction characterization with test group: Cro-TT-pRM-RBS-GFP-LVAssrAtag (BBa_K20360010).
When inserted into expression plasmid and tramsformed into E.coli. Take PETDuet-1 as an example, IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.

Fig1:Cro&pRM interaction characterization circuit

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 699