Difference between revisions of "Part:BBa J100202"

 
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<partinfo>BBa_J100202 short</partinfo>
 
<partinfo>BBa_J100202 short</partinfo>
  
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We are building indClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid.  
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We are building actClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:18, 26 September 2016


Intermediate for IndClone Red

We are building actClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 805
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 805
    Illegal AgeI site found at 678
    Illegal AgeI site found at 790
  • 1000
    COMPATIBLE WITH RFC[1000]