Difference between revisions of "Part:BBa K1920006"

 
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pBAD is an E.coli promoter that is induced by L-arabinose.In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription [1].[***]
 
pBAD is an E.coli promoter that is induced by L-arabinose.In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription [1].[***]
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However, under the higher concentration of glucose environment, pBAD will be inhibited[***]. But there is still a proper range of glucose concentration, allowing us to control the expression of the lysis.
+
Using pBAD as the promoter of lysis allows us to control lysis process.
 +
However, under the enviroment of high concentration of glucose, pBAD will be inhibited[***]. Hence the this is a promoter can be controlled with two usual substance, glucose and arabinose.
 +
Yet, in order to make glucose effective to pBADthe concentration need to be higher than 0.01%.
 +
 
  
 
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Revision as of 18:01, 25 September 2016


pBAD-RBS-lysis-B0015

pBAD is an E.coli promoter that is induced by L-arabinose.In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription [1].[***] Using pBAD as the promoter of lysis allows us to control lysis process. However, under the enviroment of high concentration of glucose, pBAD will be inhibited[***]. Hence the this is a promoter can be controlled with two usual substance, glucose and arabinose. Yet, in order to make glucose effective to pBADthe concentration need to be higher than 0.01%.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]