Difference between revisions of "Part:BBa K2036027:Design"

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===Design Notes===
 
===Design Notes===
−
none
+
 
 +
Feedback control mechanism,protein-protein interactions,protein-promoter interactions.
  
 
===Source===
 
===Source===
  
−
pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
+
Assembly
  
 
===References===
 
===References===
 +
 +
[1]Schubert R A, Dodd I B, Egan J B, et al. Cro’s role in the CI–Cro bistable switch is critical for λ’s transition from lysogeny to lytic development[J]. Genes & development, 2007, 21(19): 2461-2472.
 +
 +
[2]Halder S, Datta A B, Parrack P. Probing the antiprotease activity of λCIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH)[J]. Journal of bacteriology, 2007, 189(22): 8130-8138.
 +
 +
[3]Marr M T, Roberts J W, Brown S E, et al. Interactions among CII protein, RNA polymerase and the λ PRE promoter: contacts between RNA polymerase and the–35 region of PRE are identical in the presence and absence of CII protein[J]. Nucleic acids research, 2004, 32(3): 1083-1090.

Latest revision as of 09:10, 25 September 2016


pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 717
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 717
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 717
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 717
    Illegal AgeI site found at 2533
    Illegal AgeI site found at 2645
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3561


Design Notes

Feedback control mechanism,protein-protein interactions,protein-promoter interactions.

Source

Assembly

References

[1]Schubert R A, Dodd I B, Egan J B, et al. Cro’s role in the CI–Cro bistable switch is critical for λ’s transition from lysogeny to lytic development[J]. Genes & development, 2007, 21(19): 2461-2472.

[2]Halder S, Datta A B, Parrack P. Probing the antiprotease activity of λCIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH)[J]. Journal of bacteriology, 2007, 189(22): 8130-8138.

[3]Marr M T, Roberts J W, Brown S E, et al. Interactions among CII protein, RNA polymerase and the λ PRE promoter: contacts between RNA polymerase and the–35 region of PRE are identical in the presence and absence of CII protein[J]. Nucleic acids research, 2004, 32(3): 1083-1090.