Difference between revisions of "Part:BBa K2042000:Design"

Revision as of 17:19, 20 September 2016

Propionate CoA transferase for P. putida

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 675
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1081
Illegal BsaI site found at 1552

Design Notes

This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The sequence was codon optimized for Pseudomonas putida and one amino acid mutation (A243T) was introduce in order to produce (D)-Lactyl-CoA. For the design silent mutation was used for removing restriction site. The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.

Source

This sequence was synthetized by IDT

References

Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
http://www.ncbi.nlm.nih.gov/pubmed/19937726

Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.
http://www.ncbi.nlm.nih.gov/pubmed/23718266

@@ Line 7: / Line 7: @@
 ===Design Notes===
-This sequence was codon optimized for Pseudomonas putida and silent mutation was introduce for removing restriction site.
+This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The sequence was codon optimized for Pseudomonas putida and one amino acid mutation (A243T) was introduce in order to produce (D)-Lactyl-CoA.
+For the design silent mutation was used for removing restriction site.
 The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.