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Revision as of 11:10, 20 September 2016

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1106004

User Reviews

UNIQ01a8537d112e27df-partinfo-00000000-QINU

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HSiTAIWAN

This part works well.

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UNIQ01a8537d112e27df-partinfo-00000002-QINU

•••••

Igem BsAs 2013

Objective Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.

Methods Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).

Results As shown in the graphics below GFP production increases with the arsenite concentration.

Conclusion The promoter is sensitive to different arsenite concentrations.

Gfpinduccion.jpg

Gfpampliacion2.jpg

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