Difference between revisions of "Part:BBa K2151009:Design"

(Added info from sequencing 7/9/2016)
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===Design Notes===
 
===Design Notes===
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Sequencing before submission to registry revealed a 5bp deletion of bases 11-15 "aacgt" in the promoter pHLBA (BBa_K2151009). This does not affect either the -35 or -10 sites, and this part produces amilCP blue chromoprotein so the promoter is still able to drive expression of amilCP.
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Sequencing before submission to registry revealed a 5bp deletion of bases 11-15 "aacgt" in the promoter pHLBA (BBa_K2151000). This does not affect either the -35 or -10 sites, and this part produces amilCP blue chromoprotein so the promoter is still able to drive expression of amilCP.
  
  

Latest revision as of 15:19, 7 September 2016


pHLBA-B0034-K592009: Blue chromoprotein under control of strong constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequencing before submission to registry revealed a 5bp deletion of bases 11-15 "aacgt" in the promoter pHLBA (BBa_K2151000). This does not affect either the -35 or -10 sites, and this part produces amilCP blue chromoprotein so the promoter is still able to drive expression of amilCP.


Source

.

References