Difference between revisions of "Part:BBa K2042002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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a
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This sequence was codon optimized for Pseudomonas putida and silent mutation was introduce for removing restriction site.
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The iGEM Team Evry 2016 used this enzyme as the second enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.
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===Source===
 
===Source===

Revision as of 16:46, 4 September 2016


Pseudomonas sp. MBEL 6-19 PHA synthase 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 1339
    Illegal AgeI site found at 135
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for Pseudomonas putida and silent mutation was introduce for removing restriction site. The iGEM Team Evry 2016 used this enzyme as the second enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.

Source

a

References