Difference between revisions of "Part:BBa K2042001:Design"
RATOVOMANANA (Talk | contribs) (→Design Notes) |
RATOVOMANANA (Talk | contribs) (→Design Notes) |
||
| Line 9: | Line 9: | ||
This sequence was codon optimized for Pseudomonas putida and silente mutation was introduce for removing restriction site. | This sequence was codon optimized for Pseudomonas putida and silente mutation was introduce for removing restriction site. | ||
We used this sequence as second enzyme in order to produce | We used this sequence as second enzyme in order to produce | ||
| − | PLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere | + | PLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere. |
===Source=== | ===Source=== | ||
Revision as of 15:44, 4 September 2016
Pseudomonas resinovorans PHA synthase 1
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 87
Illegal XhoI site found at 172
Illegal XhoI site found at 1030 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1060
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 796
Design Notes
This sequence was codon optimized for Pseudomonas putida and silente mutation was introduce for removing restriction site. We used this sequence as second enzyme in order to produce PLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere.
Source
This sequence was synthetized by IDT
References
Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli.
Yang TH1, Jung YK, Kang HO, Kim TW, Park SJ, Lee SY.