Difference between revisions of "Part:BBa K2042001"

 
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<partinfo>BBa_K2042001 short</partinfo>
 
<partinfo>BBa_K2042001 short</partinfo>
  
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This is the coding sequence for a Polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer.  
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This is the coding sequence for a Polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer.
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This sequence is codon optimized for pseudomonas putida it includes four mutations which were found to increase yields of PLA (Yang et al.)
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This sequence is codon optimized for pseudomonas putida it includes four mutations which were found to increase yields of PLA.
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site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA); site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481
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The type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, E130, S325, S477, and Q481
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We note that unlike BioBrick BBa K1211002 Yale 2013, one of the mutation is not on the Serine 477 on Serine 475.  
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One of the mutation substitue the serine 477 in G differently in
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which is able to do the reaction: lactylCoA + PLA(n) = PLA(n+1).  
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We note that unlike BioBrick BBa K1211002 Yale 2013, one of the mutation is on the serine 477 not on serine 475 which is wrong.
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The enzyme is able to do the reaction: lactylCoA + PLA(n) = PLA(n+1).
  
 
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Revision as of 13:41, 4 September 2016


Pseudomonas resinovorans PHA synthase 1

This is the coding sequence for a Polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer. This sequence is codon optimized for pseudomonas putida it includes four mutations which were found to increase yields of PLA. The type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, E130, S325, S477, and Q481 One of the mutation substitue the serine 477 in G differently in We note that unlike BioBrick BBa K1211002 Yale 2013, one of the mutation is on the serine 477 not on serine 475 which is wrong. The enzyme is able to do the reaction: lactylCoA + PLA(n) = PLA(n+1).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
    Illegal XhoI site found at 172
    Illegal XhoI site found at 1030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 796