Difference between revisions of "Part:BBa J100281:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
pBAD-RBS-tetA from Part K091133
+
pBAD-RBS-tetA from Part K199029
 
sacB from Part J119042
 
sacB from Part J119042
 
linker sequence optimized from Part J119386
 
linker sequence optimized from Part J119386
  
 
===References===
 
===References===

Latest revision as of 18:31, 25 June 2016


tetA+sacB without RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 426
    Illegal BamHI site found at 2578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 980
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 2525
    Illegal AgeI site found at 2552
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Linker sequence was modified to optimize the GC content of the primers.


Source

pBAD-RBS-tetA from Part K199029 sacB from Part J119042 linker sequence optimized from Part J119386

References