Difference between revisions of "Part:BBa J100269"
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| − | In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100269 to be used as | + | In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson ''et al.'', designed part J100269 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and ''E. coli'' in a chemostat. |
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Latest revision as of 21:00, 17 June 2016
SP-T7Promiscuous_L1-192.2 (Carlson et al. 2014)
In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100269 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5475
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2016
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5475
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5475
Illegal NgoMIV site found at 8437 - 1000COMPATIBLE WITH RFC[1000]