Difference between revisions of "RFC25 "Freiburg standard" parts"
VolkerMorath (Talk | contribs) (→Reptorter proteins) |
VolkerMorath (Talk | contribs) (→Split proteins) |
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==Split proteins== | ==Split proteins== | ||
| − | + | TEV Protease | |
BBa_K1159101 | BBa_K1159101 | ||
BBa_K1159102 | BBa_K1159102 | ||
Revision as of 22:40, 14 May 2016
Contents
Reptorter proteins
Fluorescent proteins
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| Protein | Color | BioBrick | Excitation [nm] | Emission [nm] | Quantum Yield |
| GFP | Green | BBa_K648013 | 484 | 507 | 0.60 |
| sfGFP | Green | BBa_K1321337 | 485 | 510 | 0.65 |
| sYFP2 | Yellow | BBa_K1159301 | 514 | 527 | 0.61 |
| eCFP | Cyan | BBa_K1159302 | 439 | 476 | 0.40 |
| mRFP | Red | BBa_K1615089 | 584 | 607 | 0.25 |
Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.
Luciferases
| Luciferases | |||
|---|---|---|---|
| Protein | BioBrick | Size [kDa] | |
| Firefly luciferase | 61 | ||
| Renilla luciferase | 36 | ||
| NanoLuc luciferase | BBa_K1159001 | 19 | |
| Gaussia luciferase | |||
Transmembrane Domains
Linker domains
| Linker | |||
|---|---|---|---|
| Protein | Sequence | BioBrick | Length [residues] |
| Short Linker | (Gly-Gly-Ser-Gly) | BBa_K243004 | 4 |
| Middle Linker | 2x (Gly-Gly-Ser-Gly) | BBa_K243005 | 8 |
| Long Linker | 3x (Gly-Gly-Ser-Gly) | BBa_K243006 | 12 |
Catalytic domains
Erythromycin Esterase Type II BBa_K1159000
Laccase BBa_K1159002
TEV Protease BBa_K1159100
Thermonuclease BBa_K1159105
Split proteins
TEV Protease BBa_K1159101 BBa_K1159102
SpyCatcher/SpyTag BBa_K1159200 BBa_K1159201
Binding proteins
Fluorescein-Binding Anticalin BBa_K1159003
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